Capillary Electrophoresis of Oligonucleotides
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Purity and Content Analysis of Oligonucleotides by Capillary Gel Electrophoresis
Chromatography and electrophoresis are the most common techniques available for the separation of mixtures. For the analysis of large molecules, capillary gel electrophoresis (CGE) was not introduced until the late 1980s, well after most chromatographic techniques. Guttman et al.,1 Cohen et al.,2 and Kasper et al.,3 all independently, combined the principles of slab gel electrophoresis with the...
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Z. Naturforsch. 50b, 671-676 (1995); received October 12, 1994 Tentacle Polymers of PS-PEG, Oligonucleotides, Solid Phase, Capillary Electrophoresis PS-PEG tentacle polymers are modified for the synthesis of oligonucleotides covalently bound to polyethylene glycol (PEG ) at their 3'-position by the solid phase method without additional steps. The average stepwise coupling rates and overall yiel...
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We coin the term "smart aptamers" -- aptamers with predefined binding parameters (k(on), k(off), Kd) of aptamer-target interaction. Aptamers, in general, are oligonucleotides, which are capable of binding target molecules with high affinity and selectivity. They are considered as potential therapeutic targets and also thought to rival antibodies in immunoassay-like analyses. Aptamers are select...
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A unique tactic for the separation of single-base sequential isomers of oligomeric single-stranded DNA by a CE separation system employing the specific interaction of metal ion with nucleotide was demonstrated, enabling the separation of the mixture of a 12-mer oligonucleotide and its single-base mutants, as well as their positional isomers.
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5'-Phosphoramidites protected by 2-nitrophenylethyl (NPE) and 2-(4-nitrophenyl)ethoxy carbonyl (NPEOC) functions were employed for in situ synthesis of oligonucleotides in 5'-->3' direction on flat glass surfaces. By this inverse synthesis format, the oligonucleotides are attached to the solid support via their 5'-ends while the free 3'-hydroxyl groups are available as substrates for enzymatic ...
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